Project
Part:BBa_K112408:Design
Designed by: Christina Brown Group: iGEM08_UC_Berkeley (2008-10-23)
T4 phage derived lysis device
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1347
Illegal NheI site found at 1370 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 67
Illegal NgoMIV site found at 1700
Illegal AgeI site found at 953
Illegal AgeI site found at 1023 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1602
Design Notes
pBjh1601CA-K112408
T4 derived lysis device Sub into pBjh1601KA (5025 bp, EcoR1/BamH1) Product is pBjh1601KA-K112408 (EcoR1/BamH1 3196/1824 S) ----------------------------
Source
Jin Hu. JC Anderson lab, UC Berkeley