Project

Part:BBa_K112408:Design

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-23)


T4 phage derived lysis device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
    Illegal NheI site found at 1370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 67
    Illegal NgoMIV site found at 1700
    Illegal AgeI site found at 953
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1602


Design Notes

pBjh1601CA-K112408

T4 derived lysis device

Sub into pBjh1601KA		    (5025 bp, EcoR1/BamH1)

Product is pBjh1601KA-K112408      (EcoR1/BamH1 3196/1824 S)
----------------------------


Source

Jin Hu. JC Anderson lab, UC Berkeley

References